- 品名： 美國BD腦心浸出液培養基
- 品牌： 美國BD
- 型號： 237500
- 產品詳情
- 規格參數
用途:用于培養鏈球菌，肺炎鏈球菌、腦膜炎球菌等苛養菌。
成分* 含量 g/L
牛腦浸粉7.7g
牛心浸粉 9.8g
月示蛋白胨 10.0
葡萄糖 2.0
氯化鈉 5.0
磷酸二鈉 2.5
pH 7.4±0.2
Bacto? Brain Heart Infusion Approximate Formula* Per Liter
Calf Brains, Infusion from 200 g 7.7 g
Beef Heart, Infusion from 250 g 9.8 g
Proteose Peptone 10.0 g Dextrose 2.0 g
Sodium Chloride 5.0 g
Disodium Phosphate 2.5 g
可根據操作標準自行調整配方
制備方法：
溶解37g培養基至1L蒸餾水中，混勻分裝至最終容器中，121℃高壓滅菌15分鐘。
Directions：Suspend 37g of the powder in 1 L of purified water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Autoclave at 121°C for 15 minutes. Test samples of the finished product for performance using stable, typical control cultures.
Bacto? Brain Heart Infusion Dehydrated Appearance: Light tan, free-flowing, homogeneous. Solution: 3.7% solution, soluble in purified water upon boiling. Solution is light to medium amber, clear. Prepared Appearance: Light to medium amber, clear. Reaction of 3.7% Solution at 25°C: pH 7.4 ± 0.2
BHI肉湯是一種營養豐富的緩沖培養基，含有大腦和心臟組織以及胃蛋白酶，以提供蛋白質和其他必要的營養物質來支持挑剔和不挑剔的微生物的生長。在含有6.5% 氯化鈉的配方中，鹽作為一種差異和/或選擇性介質，干擾不耐鹽生物的膜通透性和滲透動力學平衡。濾絲富集法(羊血消化法)被納入一種管狀配方，用于培養流感嗜血桿菌等較為挑剔的微生物。
